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. 2022 Jan 18;10:825399. doi: 10.3389/fbioe.2022.825399

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Copyright © 2022 Park, Song, Lee, Jo, Lee, Jeon, Kwon, Kim, Baek, Kim, Yang, Kim and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mass spectrometry analysis of cell lysates. (A) mRNA expression of glycoside hydrolases (β-fructosidase, amylosucrase). The error bars indicate the standard deviation of triplicate samples. *p < 0.05, **p < 0.01. (B) MALDI-TOF-MS spectra of inulin degraded by cell lysates of pET28a-bfrA. Peaks are labeled by DP. G, glucose; F, fructose. All molecular ions are [M + Na]⁺. (C) MALDI-TOF-MS spectra of sucrose degraded by cell lysates of pET28a-ams, and pET28a-bfrA. All molecular ions are [M + Na]⁺.