Table 1.

Summary of the recent studies highlighting the impact of different media on Candida albicans containing polymicrobial biofilms.

Bacteria interacting with C. albicans	Media composition	Influence on polymicrobial biofilm	Reference
Actinomyces naeslundii	RMPI-1640 vs Artificial Saliva Medium	Biomass and metabolic activity were C. albicans strain and media specific	Arzmi et al. (2016)
Streptococcus grodonii	RPMI-1640, Todd Hewitt Broth + 0.02% Yeast Extract, 1:1 combination, synthetic saliva	Media composition influenced biofilm architecture, although all enabled synergistic interaction	Montelongo-Jauregui et al. (2016)
Streptococcus mutans	RMPI-1640 vs Artificial Saliva Medium	Biomass and metabolic activity were C. albicans strain and media specific	Arzmi et al. (2016); Brito et al. (2021)
	Different glucose concentrations	[Glucose] >60mM
		↑ biofilm biomass
		↑C. albicans CFUs/ml
		↑ Insoluble EPS