FIGURE 5.

Decreased co-occurrence network among genus bacteria in PCD patients. (A) Representative co-abundance network of genera in three groups, respectively. Based on the database of abundance of each taxon in genus, correlations among top 100 genera were calculated and correlation coefficients (p_fdr < 0.05, ∣r∣ > 0.6) were regarded as clear links and visualized by Cytoscape v3.8.2. False discovery rate (FDR) was controlled using the methods of Benjamini, Hochberg, and Yekutieli after the multiplicity testing and adjusted p_fdr < 0.05 was considered significant. Green dots represent genus bacteria and the gray line between two dots represents mutual correlation; red dots were labeled blue for 6 key diarrhea-related taxa in three groups. Gut microbiota in HC and Non-PCD groups are closely connected, but tended to assemble to several modules separately in PCD. (B) Reduced co-abundance network of Alistipes (upper panel) and augmented co-abundance network of Enterococcus (lower panel) with other genus microbes in PCD. Blue circles represent bacteria in genus, red lines denote positive correlations, and green lines indicate negative correlations.