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. 2022 Feb 18;16:843025. doi: 10.3389/fncir.2022.843025

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Copyright © 2022 Qi and Feldmeyer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acetylcholine-induced V_m changes in L4 RS, FS and nFS neurons are mainly mediated by muscarinic ACh receptors. (A) An example recording of the time course of ACh-induced Vm change under control condition and in the presence of atropine, a general mAChR antagonist, in a L4 RS neuron. (B) Same as panel (A) but for a L4 FS interneuron. (C) Same as panels (A,B) but for two L4 nFS interneurons: upper, sub-threshold depolarisation which could be completely blocked by atropine; lower, supra-threshold depolarisation which could be partly blocked by atropine. (D) Comparison histograms for L4 RS (left), FS (middle), and nFS (right) neurons under control conditions and in the presence of atropine. P value was calculated using the non-parametric Wilcoxon signed rank test. ** p < 0.01, *** p < 0.001, n.s. p ≥ 0.05.