Skip to main content
. 2022 Feb 18;10:826801. doi: 10.3389/fcell.2022.826801

FIGURE 2.

FIGURE 2

Effect of HT on mitochondrial distribution and function in porcine oocytes. (A) Representative images of functional mitochondria after HT exposure in porcine oocytes. Red, Mito-Tracker. Bar = 100 µm. (B) Representative images of Mito-Tracker and Tom20 after HT exposure in porcine oocytes, corresponding to functional mitochondria and total mitochondria, respectively. (C) The ratio of Mito-Tracker/Tom20 after HT exposure in porcine oocytes. Red, Mito-Tracker; Green, Tom20. Bar = 20 µm **, p < 0.01. (D) Representative images of the mitochondria distribution after HT exposure in porcine oocytes. Type Ⅰ, cluster pattern in the submembranous area; Type Ⅱ, granule pattern in pericortical area; Type Ⅲ, crystal pattern in peripheral area. In all three patterns, no mitochondria were detected in the center of the oocyte. Abnormal distribution: Type Ⅱ, Type Ⅲ. (E) The rate of abnormal mitochondria distribution after HT exposure. Red, Mito-Tracker. Bar = 20 µm *, p < 0.05. (F) Representative images of the JC-1 green and red channel after HT exposure in porcine oocytes. (G) The JC-1 signal (red/green ratio) after HT exposure. Bar = 100 µm *, p < 0.05.