Skip to main content
. 2022 Feb 18;10:826801. doi: 10.3389/fcell.2022.826801

FIGURE 3.

FIGURE 3

Effect of HT on ER distribution and function in porcine oocytes. (A) Representative images of the ER after HT exposure in porcine oocytes. (B) Relative fluorescence intensity of ER-Tracker after HT exposure. Green, ER-Tracker. Bar = 100 µm **, p < 0.01. (C) Representative images of the ER distribution after HT exposure in porcine oocytes. Type Ⅰ, accumulated pattern in proximity to oolemma; Type Ⅱ, diffused pattern in peripheral area, neither reached in cortex nor accumulated. Abnormal distribution: Type Ⅱ. (D) The rate of abnormal ER distribution after HT exposure. Green, ER-Tracker. Bar = 20 µm *, p < 0.05. (E) The relative mRNA expression of GRP78, ATF4, and CHOP in the control and HT-treated group. *, p < 0.05; **, p < 0.01; ***, p < 0.001. (F) Representative images of GRP78 intensity after HT exposure in porcine oocytes. Bar = 100 μm; enlarged bar = 20 µm. (G) Relative fluorescence intensity of GRP78 after HT exposure. Red, GRP78; Blue, DNA. *, p < 0.05. (H) Western blot result of the protein expression of GRP78 after HT exposure. (I) The band intensity analysis for GRP78 after HT exposure. **, p < 0.01.