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. 2022 Feb 18;10:826801. doi: 10.3389/fcell.2022.826801

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Copyright © 2022 Lee, Sun, Zhou, Jiang, Li, Heo and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HT on lysosome function in porcine oocytes. (A) Representative images of lysosomes after HT exposure in porcine oocytes. (B) Relative fluorescence intensity of Lyso-Tracker after HT exposure. Red, Lyso-Tracker. Bar = 100 µm *, p < 0.05. (C) The relative mRNA expression of LAMP2, LC3, and ATG7 in the control and HT-treated group. *, p < 0.05. (D) Representative images of LC3 intensity after HT exposure in porcine oocytes. (E) Relative fluorescence intensity of LC3 after HT exposure. Green, LC3; Blue, DNA. Bar = 20 µm **, p < 0.01. (F) Western blot result of LC3B-Ⅱ/Ⅰ protein expression after HT exposure in porcine oocytes. (G) The band intensity analysis for LC3B-Ⅱ/Ⅰ ratio after HT exposure. *, p < 0.05.