FIGURE 6.

Schematic diagram illustrating how HT disturbs meiotic maturation by inducing abnormal distribution and dysfunction of organelles in porcine oocytes. HT exposure induces abnormal distribution and dysfunction of mitochondria, with a decrease in the number of functional mitochondria and in MMP. HT exposure also causes abnormal ER distribution and induced ER stress. Moreover, HT exposure induces abnormal distribution and dysfunction of Golgi apparatus, reflected by a decrease in vesicle transporters. These results indicate an increase in damaged organelles. Moreover, an increase in lysosomal damage and autophagy after HT exposure was noted, a consequence of recycling of damaged organelles. Thus, organelles defects induced by HT exposure disturb meiotic maturation in porcine oocytes. ∆ψm; MMP: mitochondrial membrane potential.