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. 2022 Feb 18;10:826801. doi: 10.3389/fcell.2022.826801

TABLE 1.

Primer sequences used in RT-qPCR.

Gene Gene accession no. Primer sequence (5′-3′) Annealing Temp(°C) Length (bp)
Gapdh AK234838 F: AAG​TTC​CAC​GGC​ACA​GTC​AAG 60 112
R: CAC​CAG​CAT​CAC​CCC​ATT​T
BiP ( GRP78 ) J03214.1 F: ACC AAT GAC CAA AAT CGC CT 60 246
R: GTG ACT TTC CAG CCA CTC AA
ATF4 NM_001123078.1 F: TGA GCC CTG ACT CCT ATC TG 60 277
R: TCC AGC TCT TTA CAT TCG CC
CHOP NM_001144845.1 F: AAG ACC CAG GAA ACG GAA AC 60 261
R: TCC AGG AAA GGT CAG CAG TA
Lamp2 AK235422 F: GCTTTTGCAGCGTTGTGG 60 169
R: GAC​GAG​GCA​GAG​CAT​AAG​GAG
Lc3 NM_001190290 F: CCG​AAC​CTT​CGA​ACA​GAG​AG 60 206
R: AGG​CTT​GGT​TAG​CAT​TGA​GC
ATG7 AK240528 F: AGA​TTG​CCT​GGT​GGG​TGG​T 60 140
R: GGG​TGA​TGC​TGG​AGG​AGT​TG