FIGURE 3.

Western blots of mock-treated and α-L-arabinofuranosidase-digested samples from rhEPO-producing Physcomitrella lines. Ten microgram total protein of precipitated culture supernatants of the rhEPO-producing lines 174.16, Δgalt1 and X24 were digested with one unit of α-L-arabinofuranosidase (α-Arafase), while control samples were treated equivalently but without α-L-arabinofuranosidase (mock). After separation on SDS-PAGE and blotting, the PVDF-membrane was subsequently incubated with the anti-1,5-α-L-arabinan antibody (LM6-M, 1:10) (A) and an anti-hEPO monoclonal antibody (1:4,000) (B).