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. 2022 Jan 18;9(7):2102855. doi: 10.1002/advs.202102855

Figure 2.

Figure 2

Identification of putative CaV1.2‐ and CaV1.3‐antagonizing essential oil products. A) High‐throughput analysis. Independent CaV1.2‐ and CaV1.3‐specific CaB‐A systems (pCaV/pKK56/pMX125/pMX199) were depolarized with 20 mm KCl and immediately seeded into culture wells supplemented with 4 × 10−5 v/v of different plant essential oils. Data are mean FOC (fold of DMSO control) ± SD of SEAP levels scored at 48 h after exposure to essential oils (n = 3 independent experiments). B,C) Dose‐dependent validation of the most active essential oil hits. (B) CaV1.2‐ and (C) CaV1.3‐specific CaB‐A systems were depolarized with 20 mm KCl and added to culture wells containing different essential oil dilutions (v/v). Data in (B,C) are mean ± SD of SEAP levels scored at 48 h after exposure to essential oils (n = 3 independent experiments). DMSO (solvent) levels in cell culture medium were kept below 0.4%.