Figure 7.

ERK1/2‐SOD2 and ERK1/2‐ANGPTL4 are responsible for the antiapoptotic and paracrine angiogenic effects of irisin‐induced ADSCs, respectively. A,B) Western blots and quantification of cleaved caspase‐3 protein expression in ADSCs. C) Representative images of TUNEL‐positive ADSCs (red) with or without H₂O₂ (200 µm, 6 h). Cell nuclei were stained with DAPI (blue). D) Quantification of TUNEL‐positive ADSCs. ADSCs were transfected with siRNA against SOD2 (siSOD2) or siRNA against ANGPTL4 (siANGPTL4) 36 h before irisin treatment (100 ng mL⁻¹, 24 h). U0126: 10 µm, 2 h before irisin treatment. n = 4. E,F) Tube formation of rCAECs treated with F12, F12+ANGPTL4 (2 µg mL⁻¹), ADSC‐vehicle‐CM, ADSC‐irisin‐ANGPTL4^WT‐CM, or ADSC‐irisin‐ANGPTL4^KD‐CM. n = 4. ANGPTL4^WT, ANGPTL4 scRNA; ANGPTL4^KD, ANGPTL4 siRNA. The data were analyzed by 1‐way ANOVA, followed by a Bonferroni post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001. ns, not significant.