Figure 8.

Irisin increases ANGPTL4, CSF2RB, and SOD2 expression in ADSCs via integrin αV/β5. A,B) Quantitative PCR analysis of mRNA expression of integrin αV, β5, and β1 (A) and Angptl4, Csf2rb, and Sod2 (B) in rat ADSCs. ADSCs were transfected with scRNA, siRNAs against integrin αV and β5 (αVβ5^KD), or siRNAs against integrin αV and β1 (αVβ1^KD) for 36 h and then treated with irisin (100 ng mL⁻¹) for another 24 h. n = 3–4. C,D) Western blots and quantification of integrin αV, β5, and β1, ANGPTL4, CSF2RB, and SOD2 protein expression in rat ADSCs. ADSCs were transfected with scRNA, siRNAs against integrin αV and β5 (αVβ5^KD), or siRNAs against integrin αV and β1 (αVβ1^KD) for 36 h and then treated with irisin (100 ng mL⁻¹) for another 48 h. n = 3–4. E,F) Western blots and quantification of integrin αV and β5, ANGPTL4, CSF2RB, and SOD2 protein expression in mouse ADSCs. ADSCs were transfected with scRNA or siRNAs against integrin αV and β5 (αVβ5^KD) for 36 h and then treated with irisin (100 ng mL⁻¹) for another 48 h. n = 3–4. The data were analyzed by 1‐way ANOVA, followed by a Bonferroni post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant.