Figure 3.

Demonstration of the occurrence of a cascade reaction initiated by exposure of a GOx/hemin‐loaded G₄‐hydrogels based on TMB oxidation. a) Overview of the cascade reactions occurring. b) UV–vis absorption spectra of a TMB solution in the presence (4 h) of differently loaded G₄‐hydrogels and glucose (2 g L⁻¹). c) UV–vis absorption spectra of a TMB solution in presence of a GOx/hemin‐loaded G₄‐hydrogel and glucose (2 g L⁻¹) for 0–4 h. d) UV–vis absorption at 450 nm of a TMB solution in presence of a GOx/hemin‐loaded G₄‐hydrogel at different glucose concentrations as a function of time. All data in panel (d) were expressed as means ± standard deviations over triplicate experiments with separately prepared hydrogels. Asterisks indicate statistical significance at **p < 0.01 and ****p < 0.0001 (one‐way ANOVA test) between differences with respect to exposure to the hydrogel with the highest glucose loading (5 g L⁻¹).