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Co-infected flea plating schematic. The flea triturate is mixed with molten agar and plated as a top layer on an agar plate. For molten agar containing no selective antibiotic, both strains (open circles for antibiotic resistance marker encoding strain, closed circles for unmarked strain) grow providing a total CFU count per flea while selective antibiotic containing media allows for CFU enumeration of the strain that contains an antibiotic selective marker
