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. Author manuscript; available in PMC: 2022 Mar 4.
Published in final edited form as: Brain Behav Immun. 2020 Feb 22;87:610–633. doi: 10.1016/j.bbi.2020.02.006

Figure 1:

Figure 1:

Sterile lung injury (SLI) promotes microglia/macrophage morphologic changes throughout the dorsomedial brainstem

A1. Representative images of coronal brainstem sections from saline- (orange) and Bleo-treated rats (red) immunostained for Iba-1 and amplified using avidin/biotin + 3,3’-diaminobenzadine (DAB). DAB visualized Iba-1+ IR was used to count cells in the dorsal brainstem.

A2. Magnified representative images of Iba-1+ cells in the dorsal border of the Central Canal (dbCC, left panel) and Area Postrema (AP, right panel).

B1. The number of Iba-1+ cells in the czAP was not significantly different in Bleo-compared to saline-treated rats (P = 0.87, Two-tail t-Test).

B2. The branch number (Br#) in the czAP decreased in Bleo-compared to saline-treated rats (P = 0.024, Two-tail t-Test).

B3. Bleo-treated rats had fewer branch endpoints (BEPs) in the czAP compared to saline-treated rats. (P = 0.03, Two-tail t-Test).

B4. The total branch length (TBL) in the czAP was reduced in Bleo-compared to saline-treated rats (P = 0.031, Two-tail t-Test).

C1. The number of Iba-1+ cells in the dorsal border of the CC (dbCC) increased in Bleo- (n = 7) compared to saline- (n = 8) treated rats (P = 0.018, Two-tail t-Test).

C2. The BN#/Iba-1+ cell (Br#) located in the dbCC increased in Bleo-compared to saline-treated rats (P = 0.027, Two-tail t-Test).

C3. The number of BEPs/Iba-1+ cell (BEPs) in the dbCC increased in Bleo-compared to saline-treated rats (P = 0.049, Two-tail t-Test).

C4. The TBL/Iba-1+ cell in the dbCC increased in Bleo-compared to saline-treated rats (P = 0.035, Two-tail t-Test).

D1. The number of Iba-1+ cells in the Dorsal Motor Nucleus of the Vagus (DMNX) was not significantly different between Bleo- and saline-treated rats (P = 0.53, Two-tail t-Test).

D2. The Br# in the DMNX was reduced in Bleo-compared to saline-treated rats (P = 0.01, Two-tail t-Test).

D3. Bleo treated rats exhibited reduced BEPs in the DMNX in comparison to saline-treated rats (P = 0.01, Two-tail t-Test).

D4. The TBL in the in the DMNX was reduced in Bleo-compared to saline-treated rats (P = 0.012, Two-tail t-Test).