Table. 1:
Primary and secondary antibodies utilized in the current study.
| 1° Antibody | Species | Dilution | Manufacturer | Product # |
|---|---|---|---|---|
|
| ||||
| COX-2 | Goat (Gt) | 1: 1000 | Abcam | ab23672 |
| GFAP | Chicken (Ch) | 1 : 1000 | Abcam | ab4674 |
| lba-1 | Rabbit (Rb) | 1 : 1000 | Wako | 019-19741 |
| IL-1 1β/Pro-IL-1β | Rb | 1 : 500 | Abcam | ab9722 |
| IL-6 | Gt | 1 : 500 | R&D | AF506 |
| MAP-2 | Rb | 1 : 5000 | Abcam | ab32454 |
| NeuN Alexa® Fluor 647 | Rb | 1 : 1000 | Abcam | ab190565 |
| TNF-α | Gt | 1 : 2000 | R&D | AF-510-NA |
| VGIuT2 | Guinea Pig (GP) | 1 : 2000 | Millipore | AB2251-I |
|
| ||||
| 2° Antibody | Species | Dilution | Manufacturer | Product # |
|
| ||||
| Anti-Ch Alexa® Fluor 488 | Donkey (Dk) | 1 : 1000 | Jackson ImmunoResearch (JIR) | 703-545-155 |
| Anti-Gt Alexa® Fluor 647 | Dk | 1: 1000 | JIR | 705-295-003 |
| Anti-Gt Biotinylated Bt | Dk | 1 : 5000 | JIR | 705-065-003 |
| Anti-GPTRITC | Dk | 1 : 1000 | JIR | 706-545-148 |
| Anti-Rb Alexa® Fluor 488 | Dk | 1 : 1000 | JIR | 711-475-152 |
| Anti-Rb Alexa® Fluor 647 | Dk | 1 : 1000 | JIR | 711-295-152 |
| Anti-Rb Bt | Dk | 1 : 5000 | JIR | 711-065-152 |
Abbreviations are as follows: Cyclooxygenase-2 (COX-2); Glial fibrillary acidic protein (GFAP); Ionized calcium-binding adapter molecule (lba-1); lnterleukin-1 beta (IL-1β); lnterleukin-6 (IL-6); Microtubule associated protein 2 (MAP2); Neuronal Nuclei (NeuN);Tumor necrosis factor-alpha (TNF-α); vesicular glutamate transporter 2 (VGIuT2).