TABLE 1.
Interference with LAC replication due to dsSIN-expressed sequencesa
| dsSIN insertb | Mean LAC titer (SD) (log10 TCID50/ml)c at:
|
|||
|---|---|---|---|---|
| 24 h | 48 h | 72 h | 96 h | |
| None (TE/3′2J) | 4.5 (0.3) | 5.6 (0.3) | 5.9 (0.3) | 5.9 (0.3) |
| LAC M segment RNA inserts | ||||
| nt 1–1040 (G2 gene) | 4.3 (0.4) | 5.9 (0.3) | 5.6 (0.3) | 5.9 (0.3) |
| Antisense 1–1040 | 3.2 (0.6) | 5.0 (0.5) | 5.2 (0.6) | 5.3 (0.3) |
| nt 1021–2453 (NSm and 5′ G1 genes) | 4.9 (0.3) | 5.9 (0.3) | 5.9 (0.3) | 6.3 (0.0) |
| nt 2454–3625 (G1 gene center) | 4.3 (0.0) | 4.3 (0.3) | 5.3 (0.3) | 4.9 (0.3) |
| Antisense 2454–3625 | 4.6 (0.3) | 5.3 (0.4) | 4.9 (0.3) | 5.3 (0.4) |
| nt 3621–4495 (3′ G1 gene) | 5.3 (0.4) | 5.6 (0.3) | 6.3 (0.0) | 5.9 (0.3) |
| Antisense 3621–4495 | 2.7 (0.3) | 6.0 (0.5) | 6.3 (0.3) | 6.2 (0.6) |
| LAC S segment RNA inserts | ||||
| Full-length S | 1.9 (1.7) | 2.3 (1.9) | 2.8 (2.0) | 2.7 (2.3) |
| Antisense S | 0.9 (0.9) | 1.3 (1.4) | 0.9 (1.2) | 0.6 (1.8) |
| Antisense complementary termini | 1.7 (0.8) | 3.5 (0.9) | 4.3 (0.4) | 4.3 (0.8) |
| Nonstructural gene | 3.7 (0.3) | 4.8 (0.4) | 5.1 (0.8) | 5.2 (0.8) |
| Antisense nonsructural gene | 1.3 (0.6) | 3.3 (0.3) | 4.2 (0.6) | 4.2 (0.6) |
b
A. albopictus C6/36 mosquito cells were infected with recombinant dsSIN containing the insert shown at a MOI of 20 to 50. After 48 h, cell cultures were challenged with LAC at a MOI of 0.01.
c
The infectious LAC titer was determined in medium removed every 24 h. Boldface numbers indicate LAC titers >1.7 log10 lower than the LAC titer in nonrecombinant SIN (TE/3′2J)-infected control cultures at the same time point and are considered to indicate significant interference.