Viability of (a,b) HT29
and (c), (d) SW480 cells treated with AIS-2MPA-3,
AIS-2MPA-3-30%ALA, and AIS-2MPA-3-50%ALA QDs and (e,f) HT29 and (g,h)
SW480 cells treated with AIS-PEI/2MPA-1, AIS-PEI/2MPA-1-30%ALA, and
AIS-PEI/2MPA-1-50%ALA QDs after 24 h incubation with and without PDT
measured by Alamar blue compared to the untreated cells. Viability
of 0.35 mM ALA of AIS-2MPA-3, AIS-2MPA-3-30%ALA, and AIS-2MPA-3-50%ALA
QDs on (i) HT29 and (j) SW480 3D-spheroid models and 0.015 mM ALA
of AIS-PEI/2MPA-1, AIS-PEI/2MPA-1-30%ALA, and AIS-PEI/2MPA-1-50%ALA
on (k) HT29 and (l) SW480 3D-spheroid models treated with QDs and
illuminated at 420 nm blue light for 5 min. PDT control indicated
cells treated only with 420 nm irradiation for 5 min. The data are
expressed as mean ± S.D. using two-way ANOVA with Tukey’s
multiple comparison test (n = 3), (p < 0.05).