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Evaluation of interference signals and different amount of proteases for on-bead digestion. (A) Base peak chromatogram of digesting streptavidin magnetic beads with no input proteins. The interference percentages were generated in comparison with an actual APEX proteomics experiment. (B) Optimization of the amount of trypsin/Lys-C mix for on-bead digestion with 250 μL of streptavidin magnetic beads and corresponding input protein amount based on the bead titration assay.
