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. 2022 Mar;192(3):484–502. doi: 10.1016/j.ajpath.2021.11.008

Figure 6.

Figure 6

Leptin increases inflammation of the liver in multidrug resistance protein 2 knockout (Mdr2KO) mice. A–C: Gene expression of proinflammatory cytokines IL-1β (A), IL-6 (B), and C-C motif chemokine ligand 2 (CCL2; C) was assessed in liver samples from Mdr2KO and FVB/NJ (FVBN) mice treated with vehicle or leptin. D: CCL2 level was assayed in serum samples of mice treated with vehicle or leptin. E: The amount of CD11b+ macrophages was quantified by image analysis of images obtained by immunohistochemistry (IHC) for CD11b in liver samples from Mdr2KO and FVBN mice treated with leptin versus vehicle. F: Representative images of IHC for CD11b. F: Arrows point to CD11b+ cells. N = 6 (A–E). ∗P < 0.05 for leptin versus vehicle; †P < 0.05 for Mdr2KO versus FVBN mice. Scale bars = 100 μm (F).