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. 2022 Mar;192(3):484–502. doi: 10.1016/j.ajpath.2021.11.008

Figure 8.

Figure 8

Leptin-neutralizing antibody (Leptin-Ab) reduces liver fibrosis in multidrug resistance protein 2 knockout (Mdr2KO) mice. A: Immunohistochemistry (IHC) of leptin receptor (LepR) in livers of Mdr2KO and FVB/NJ (FVBN) mice treated with vehicle or Leptin-Ab. Desmin, α-smooth muscle actin (α-SMA), and collagen were assessed to measure hepatic fibrosis in male and female Mdr2KO and FVBN mice treated with vehicle or Leptin-Ab. A: Arrows point to cells expressing LepR. B–D: Desmin: mRNA (B), IHC images (C), and quantifications from image analysis (D). E–G: α-SMA: mRNA (E), IHC images (F), and quantification (G). C and F: Arrows indicate stellate cells expressing desmin and α-SMA, respectively. H–J: Collagens: collagen type 1A1 (Col1A1) mRNA (H), Sirius Red staining images (I), and quantification (J). N = 6 (B, D, E, G, H, and J). ∗P < 0.05 for leptin-Ab versus vehicle; †P < 0.05 for Mdr2KO versus FVBN mice. Scale bars = 100 μm (A, C, F, and I). C, cholangiocyte; H, hepatocyte; S, hepatic stellate cell.