Figure 9.

Leptin-neutralizing antibody (Leptin-Ab) reduces fibrogenic and proinflammatory genes in multidrug resistance protein 2 knockout (Mdr2KO) mice. A–D: The mRNA expression of transforming growth factor (TGF)-β1 fibrogenic gene (A) and of IL-1β (B), IL-6 (C), and C-C motif chemokine ligand 2 (CCL2) proinflammatory genes (D) was assessed in male and female Mdr2KO and FVB/NJ (FVBN) mice. E: CCL2 was assessed in serum of Mdr2KO and FVBN mice treated with vehicle or Leptin-Ab. F: Representative images of immunohistochemistry (IHC) for CD11b⁺ macrophages in liver sections. G: Image analysis results of CD11b IHC in liver samples. Arrows in IHC images point to cells expressing the protein of interest. N = 6 (A–E and G). ∗P < 0.05 for leptin-Ab versus vehicle; ^†P < 0.05 for Mdr2KO versus FVBN mice. Scale bars = 100 μm (F).