Fig. 3.

PTEN upregulated the expression of p21 in pancreatic cancer. (A,B) Volcano plot (A) and heatmap (B) to show the differential expressed genes of SW 1990 cells infected with sh‐Control or sh‐PTEN (n = 3). The green points represented the downregulated genes, while red points represented the upregulated genes. (C) Heatmap to show a subset of PTEN knockdown regulated genes participated in p53 pathway of SW 1990 cells (n = 3). (D,E) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with sh‐Control or sh‐PTENs were harvested for RT‐PCR analysis (d) and western blotting analysis (e). GAPDH served as an internal reference and repeated for three replicates. Data are shown as means ± SD (n = 3). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. ***P < 0.001. (F,G) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with pcDNA3.1 or PTEN plasmids were harvested for RT‐PCR analysis (F) and western blotting analysis (G). GAPDH served as an internal reference and repeated for three replicates. Data are shown as means ± SD (n = 3). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. ***P < 0.001. p21: Cyclin‐dependent kinase inhibitor 1A, also symboled as CDKN1A; GAPDH: Glyceraldehyde‐3‐phosphate dehydrogenase.