Fig. 4.

USP22 induced the p21 expression via PTEN in pancreatic cancer. (A,B) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with sh‐Control or sh‐USP22s were harvested for RT‐PCR analysis (A) and western blotting analysis (B). GAPDH served as an internal reference and repeated for three replicates. Data are shown as means ± SD (n = 3). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. ns, not significant; ***P < 0.001. (C,D) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with pcDNA3.1 or USP22 plasmids were harvested for RT‐PCR analysis (C) and western blotting analysis (D). GAPDH served as an internal reference and repeated for three replicates. Data are shown as means ± SD (n = 3). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. ns, not significant; ***P < 0.001. (E) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with or without USP22 plasmids and infected with or without sh‐PTEN were harvested for western blotting analysis. GAPDH served as an internal reference and repeated for three replicates. (F) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with or without sh‐USP22 and infected with or without sh‐PTEN were harvested for western blotting analysis. GAPDH served as an internal reference and repeated for three replicates. p21: Cyclin‐dependent kinase inhibitor 1A, also symboled as CDKN1A; GAPDH: Glyceraldehyde‐3‐phosphate dehydrogenase.