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. 2021 Nov 16;16(5):1200–1217. doi: 10.1002/1878-0261.13137

Fig. 5.

Fig. 5

USP22 upregulated the expression of p21 via PTEN‐MDM2‐p53 signaling pathway in pancreatic cancer. (A) Bubble chart to show the KEGG enriched pathways of SW 1990 cells infected with sh‐PTEN. (B) Dot chart to show the mRNA expression level of p53 and MDM2 genes in SW 1990 cells infected with sh‐Control or sh‐PTEN. Data are shown as means ± SD (n = 3, P = 0.0136 and P = 0.126, respectively). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. (C,D) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with sh‐Control or sh‐PTENs were harvested for RT‐PCR analysis (C) and western blotting analysis (D). GAPDH served as an internal reference and repeated for three replicates. Data are shown as means ± SD (n = 3). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. ns, not significant; ***P < 0.001. (E,F) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with pcDNA3.1 or PTEN plasmids were harvested for RT‐PCR analysis (E) and western blotting analysis (F). GAPDH served as an internal reference and repeated for three replicates. Data are shown as means ± SD (n = 3). Statistical analyses were performed with one‐way ANOVA followed by Tukey's multiple comparison's tests. ns, not significant; ***P < 0.001. (G) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with or without PTEN plasmids and infected with or without sh‐MDM2 were harvested for western blotting analysis. GAPDH served as an internal reference and repeated for three replicates. (H) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with or without sh‐PTEN and infected with or without sh‐MDM2 were harvested for western blotting analysis. GAPDH served as an internal reference and repeated for three replicates. (I) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with or without USP22 plasmids and infected with or without sh‐PTEN were harvested for western blotting analysis. GAPDH served as an internal reference and repeated for three replicates. (J) Forty‐eight hours postinfection, SW 1990 and HPAC cells infected with or without sh‐USP22 and infected with or without sh‐PTEN were harvested for western blotting analysis. GAPDH served as an internal reference and repeated for three replicates. p21: Cyclin‐dependent kinase inhibitor 1A, also symboled as CDKN1A; GAPDH: Glyceraldehyde‐3‐phosphate dehydrogenase.