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. 2022 Feb 18;23(4):128. doi: 10.3892/ol.2022.13248

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — 4-AAQB downregulates the expression of the PI3K, Akt and Vps34 proteins in MiaPaCa-2 and MiaPaCa-2^GEMR cells. (A) The levels of P-PI3K, PI3K, p-Akt and Akt were analyzed using western blotting. (B) Semi-quantification of p-PI3K/PI3K protein levels. (C) Semi-quantification of p-Akt/Akt protein levels. (D) The expression of Vps34 was analyzed using western blotting. (E) Semi-quantification of Vps34 protein levels. *P<0.05 and **P<0.01 vs. the untreated control cells. 4-AAQB, 4-Acetylantroquinonol B; GEMR, gemcitabine-resistant; p-, phosphorylated; Vps34, phosphatidylinositol 3-kinase catalytic subunit type 3.