Figure 7.

4-AAQB treatment leads to cellular ROS accumulation. MiaPaCa-2 and MiaPaCa-2^GEMR cells were treated with the indicated concentrations of 4-AAQB and GEM for 48 h. The ROS levels of (A) MiaPaCa-2 and (B) MiaPaCa-2^GEMR cells were measured using a DCFH-DA probe. *P<0.05 and **P<0.01 vs. the untreated control cells; ^##P<0.01 and ^###P<0.001 vs. the gemcitabine treated cells; ^@P<0.05 vs. the 4-AAQB treated cells. 4-AAQB, 4-Acetylantroquinonol B; GEMR, gemcitabine-resistant; ROS, reactive oxygen species.