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. 2022 Feb 18;23(4):128. doi: 10.3892/ol.2022.13248

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — 4-AAQB downregulates VEGFA expression and inhibits cell migration. (A) The expression of the VEGFA protein was analyzed using western blotting. (B) Semi-quantification of VEGFA protein levels. (C and E) Cell migration was assessed using a wound-healing assay. Multiple images were captured of each well at various time points. Magnification, ×100. (D and F) The migration distances were recorded and calculated using ImageJ software. *P<0.05 **P<0.01, and ***P<0.001 vs. the untreated control cells; ^#P<0.05 and ^##P<0.01 vs. the gemcitabine treated cells; ^@P<0.05 vs. the 4-AAQB treated cells. 4-AAQB, 4-Acetylantroquinonol B; GEMR, gemcitabine-resistant.