Fig. 7.

Interdependence of Gal‐1 and SCD1 expression in human HCC cell lines suggests regulation of lipid synthesis. (A) siRNA‐mediated Gal‐1 silencing elicited a decrease in SCD1 protein levels in the human HCC cell lines PLC/PRF/5, HLF, and Snu182 as exemplified in western blot analyses (left panel). Molecular weights of observed bands are noted. Quantification of yielded bands was performed (right panel). (B) In contrast, siRNA‐mediated Gal‐1 silencing did not yield a regulation of other important lipogenic enzymes, such as ACLY, FASN, and ACAC. At the same time, a tendency for proliferator‐activated receptor gamma (PPARɣ) reduction could be observed in PLC and Snu182 cell lines. n(PLC/PRF/5) = 3 cell culture experimental repeats with 3 cell culture replicates + 1 cell culture experimental repeat with 6 cell culture replicates. n(HLF, Snu182) = 3 cell culture experimental repeats with 3 cell culture replicates. (C) 20 µm OTX008 treatment for 24 h likewise led to a decrease in SCD1 in PLC/PRF/5 and HLF cell lines as demonstrated by Western blot (left panel). Molecular weights of observed bands are noted. Quantification of yielded bands was performed (right panel). Intensities were normalized to the mean of control for all tested proteins. Tukey method box‐and‐whisker plots are displayed (right panels). Mann–Whitney tests were employed; ns, not significant. All pairwise comparisons in the right panel in (B) were not significant, except for the one displayed.