Table 1.

Overview of experimental groups and timepoints.

	1	2	3	4	5	6	7	8	9	10
	Experimental groups				Control groups
Strain (RASSF1A KO or WT)	KO	KO	WT	WT	KO	WT	KO	WT	KO	WT
Injected construct	H1047R	E545K	H1047R	E545K
1× PBS injection					X	X
EV							X	X
untreated									X	X
Animals per timepoint (N)
1 week	10	10	10	10	5	5	5	5	5	5
1 month	10	10	10	10	5	5	5	5	5	5
3 months	10	10	10	10	5	5	5	5	5	5
6 months	10	10	10	10	5	5	5	5	5	5
9 months	10	10	10	10	5	5	5	5	5	5
12 months	10	10	10	10	5	5	5	5	5	5
Sum of animals per experimental group	60	60	60	60	30	30	30	30	30	30
Sum of all animals	420