Table 2.
Antibodies used for immunohistochemistry and western blots.
| Application | Dilution | Company | Catalog number |
|---|---|---|---|
| Immunohistochemistry | |||
| p110α (PI3K) | 1 : 100 | Cell Signaling Technology, Inc. | 4249 |
| Phospho‐Akt (Ser473) | 1 : 100 | Cell Signaling Technology, Inc. | 3787 |
| COX2 | 1 : 300 | Cell Signaling Technology, Inc. | 12282 |
| CPS1 | 1 : 100 | Abcam, Cambridge, UK | ab129076 |
| CK7 | 1 : 100 | Abcam | ab181598 |
| Desmin | 1 : 1000 | Abcam | Ab15200 |
| pERK 1/2 | 1 : 100 | Cell Signaling Technology, Inc. | 4370 |
| FASN | 1 : 100 | BD Biosciences, San Jose, CA, USA | 610962 |
| Gal‐1 | 1 : 100 | Abcam plc. | ab138513 |
| HA‐Tag | 1 : 100 | Cell Signaling Technology, Inc. | 3724 |
| Ki67 | 1 : 100 | Bethyl Laboratories, Montgomery, TX, USA | IHC‐00375 |
| SCD1 | 1 : 100 | Cell Signaling Technology, Inc. | 2794 |
| a‐sma | 1 : 200 | Abcam plc. | ab5694 |
| ZIP4 | 1 : 100 | ProteinTech Group, Inc., Rosemont, IL, USA | 20625‐1‐AP |
| Western blot | |||
| ACAC | 1 : 500 | Cell Signaling Technology, Inc. | 3676 |
| ACLY | 1 : 500 | Cell Signaling Technology, Inc. | 4332s |
| pAKT (S473) | 1 : 1000 | Cell Signaling Technology, Inc. | 4060 |
| pAKT (S473) | 1 : 1000 | ProteinTech Group, Inc. | 66444‐1‐Ig |
| pAKT (S308) | 1 : 1000 | Cell Signaling Technology, Inc. | 13038 |
| t‐AKT | 1 : 1000 | Cell Signaling Technology, Inc. | 9272 |
| Pan‐AKT | 1 : 1000 | Cell Signaling Technology, Inc. | 4691 |
| COX2 | 1 : 1000 | Cell Signaling Technology, Inc. | 12282 |
| pERK 1/2 (Thr202/Tyr204) | 1 : 1000 | Cell Signaling Technology, Inc. | 4370 |
| ERK1/2 | 1 : 1000 | Cell Signaling Technology, Inc. | 4695 |
| FASN | 1 : 1000 | Santa Cruz Biotechnology, Inc. | sc‐55580 |
| Gal‐1 | 1 : 1000 | Abcam plc. | ab138513 |
| Gal‐3 | 1 : 1000 | Abcam plc. | ab76245 |
| GAPDH | 1 : 2000 | Cell Signaling Technology, Inc. | 5174 |
| HA‐tag | 1 : 1000 | Cell Signaling Technology, Inc. | 2367 |
| PIK3CA | 1 : 1000 | Cell Signaling Technology, Inc. | 4249 |
| PPARγ | 1 : 500 | Cell Signaling Technology, Inc. | 2435 |
| SCD1 | 1 : 1000 | Cell Signaling Technology, Inc. | 2794 |
| p‐SGK3 (Thr320) | 1 : 1000 | Cell Signaling Technology, Inc. | 5642 |
| SGK3 | 1 : 1000 | Cell Signaling Technology, Inc. | 8156 |
| p‐STAT3 (Tyr705) | 1 : 1000 | Cell Signaling Technology, Inc. | 9145 |
| STAT3 | 1 : 1000 | Cell Signaling Technology, Inc. | 5904 |
| ZIP4 | 1 : 1000 | ProteinTech Group | 20625‐1‐AP |
| β‐Actin | 1 : 1000 | Cell Signaling Technology | 3700 |
| HRP, goat anti‐mouse IgG (secondary antibody) | 1 : 20 000 | Abbkine Scientific Co., Ltd., Wuhan, China | A21010 |
| HRP, goat anti‐rabbit IgG (secondary antibody) | 1 : 20 000 | Abbkine Scientific Co. | A21020 |