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. 2022 Feb 17;13:806837. doi: 10.3389/fphar.2022.806837

FIGURE 1.

FIGURE 1

Identification of proliferative activities of phthalide derivatives 1 to 9 and the optimal working concentration of candidate compounds for in vitro expansion of human UCB CD34+ cells. (A) The structures of phthalide derivatives 1 to 9 and the corresponding absolute number of CD34+CD38− cells after a serum-based 7-d culture in gradient concentrations as specified (n = 6). (B) The percentages of CD34+CD38− and CD34+CD49f+ subpopulations in CD34+ cells after a serum-based 7-d culture supplemented with DMSO (0.05%, v/v), SR1 (1 µM), Levistilide A (LA, 2.5 –40 µM), or Tetrahydrophthalide Neocnidilide (TN, 2.5 –40 µM) (n = 5). (C) Representative FACS profiles of CD34+CD38− and CD34+CD49f+ subpopulations in cultured CD34+ cells as described in (B). All data represent the means ± SD. Compared with fresh unless specified. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns = not significant by two-tailed unpaired Students’ t-test.