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. 2022 Mar 3;11(1):73–87. doi: 10.1093/stcltm/szab008

Figure 2.

Figure 2.

Safranin O (A-D) and type I/II collagen (E-H) staining of sectioned mouse joints. Dashed lines indicate where full-thickness defects were created. Red/purple staining for safranin O on the left indicates the presence of proteoglycans. Green and red staining on the right indicate the presence of type I and II collagen, respectively. Saline conditions showed no pronounced staining for either proteoglycans or type II collagen indicating the formation of fibrocartilage. EV and CCM conditions displayed positive staining for proteoglycans and type II collagen, with EVs providing the most prominent results (CCMH = day 6 CCM, CCML = day 3 CCM). Cartilage repair scoring showed that EV and CCML treatment groups demonstrated increased repair versus saline-treated controls, while CCMH had no effect (n = 3). ∗P < .05, ∗∗∗P < .001. Scale bars = 100 µM. Abbreviations: CCM, concentrated conditioned medium; EV, extracellular vesicle.