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. 2022 Mar 3;11(1):73–87. doi: 10.1093/stcltm/szab008

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Alcian blue, type I/II/X collagen, and DAPI staining for chondrogenic pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel (n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, mesenchymal progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.