Fig. 5.

Hsa_circ_0000277 targeted miR-873-5p to regulate tumor process and DDP resistance in ESCC cells. A, B The relative level of miR-873-5p was detected via RT-qPCR in ESCC tissues (A) and cells (B). C Transfection efficiency of anti-miR-873-5p was assessed by RT-qPCR. D The miR-873-5p quantification was performed by RT-qPCR after EC9706 and KYSE30 cells were transfected with sh-NC, sh-circ_0000277#1, sh-circ_0000277#1+anti-NC, or sh-circ_0000277#1+anti-miR-873-5p. E–I Tumor progression was analyzed using cell proliferation by CCK-8 (E, F), clonal ability by colony formation assay (G, H), and cell cycle by flow cytometry (I) in four transfection groups. J–N CCK-8 and flow cytometry were respectively used to measure the IC50 of DDP (J–L) and cell apoptosis (M, N) after DDP treatment and the above transfection. *P < 0.05