Fig. 7.

MiR-873-5p was identified as an inhibitor in ESCC development and DDP resistance by downregulating SOX4. A, B The RT-qPCR and western blot were conducted for the analysis of SOX4 mRNA level in ESCC tissues (A) and protein level in ESCC cells (B). C After transfection of pcDNA or SOX4 vector, the protein expression of SOX4 was determined via western blot. D–H ESCC progression was evaluated by examining cell proliferation via CCK-8 (D, E), colony formation capacity via colony formation assay (F, G), and cell cycle progression via flow cytometry (H) following transfection of miR-NC, miR-873-5p, miR-873-5p+pcDNA, or miR-873-5p+SOX4. I–M After the above transfection under the treatment of DDP, IC50 of DDP (I–K) and cell apoptosis rate (L–M) were respectively examined via CCK-8 and flow cytometry. *P < 0.05