Table 3.
Primers used in the present study to estimate host and bacterial cell numbers
|
Target (gene) |
Primer name |
Primer sequence |
Annealing temperature (°C) |
Product size (nt) |
References |
|---|---|---|---|---|---|
|
E. diaphana (Ef1-α) |
Ef1-α-fwd |
AGCACTGAGCCACCATACAG |
60 |
88 |
[107] |
|
Ef1-α -rev |
TTGGGTTATAGCCGGTCTTC |
60 |
[107] |
||
|
A. salina (ß-actin) |
art-actin-fwd |
GGTCGTGACTTGACGGACTATCT |
60 |
147 |
[108–111] |
|
art-actin-rev |
AGCGGTTGCCATTTCTTGTT |
60 |
[108–111] |
||
|
Universal bacteria (conserved inter V2–V3 16S rRNA gene region) |
259-fwd |
GGTAAHRGCYYACCAAG |
54 |
98 |
[112] |
|
357-rev |
CTGCTGCCTCCCGTAGGAG |
54 |
Reverse complement of ‘primer 1’ [113] |