Fig. 2.

Flavivirus antibody reactivity patterns. Reciprocal PRNT₉₀-specific endpoint titres for the African ZIKV lineage strain (ZIKV_AF), the Asian ZIKV lineage strain (ZIKV_AS), SPOV, DENV-2, YFV, WSLV, WNV, SLEV, ROCV and BSQV of ZIKV among ECLIA-positive sera in pets and peri-domestic animals in Brazil (a), in Côte d’Ivoire (b) and in NHP from Côte d’Ivoire (c). 2D antigenic cartography showing neutralizing activity against related flaviviruses among domestic animals in Brazil (d), Côte d’Ivoire (d) and in NHP in Côte d’Ivoire (e). Each unit of antigenic distance (length of one square grid side, measured in any direction) is equivalent to a fourfold dilution in the PRNT₉₀. Each circle corresponds to one tested serum sample showing titres (sera with a negative PRNT₉₀ or an endpoint titre <10 are not shown) and circle size suggests intra-sample differences. Grey circles indicate the antigens or antisera (tested viruses). In (d), titers obtained with the African ZIKV lineage strain were used for animals from Côte d’Ivoire and with the Asian ZIKV lineage strain for animals from Brazil. Horizontal lines plotted in (a), (b) and (c) show median