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. 2022 Feb 11;12:788040. doi: 10.3389/fmicb.2021.788040

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Copyright © 2022 Yue, Wang, Shi, Xie, Li, Guan, Evivie, Liu, Li and Huo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DEG analysis of HT-29 cells treated with L. acidophilus (n = 3). (A) The volcano plot of a differential gene. (B) Clustering heatmap of differentially expressed gene expression. Controls 1, 2, and 3 were nontreated with L. acidophilus in HT-29 cells. Samples 1, 2, and 3 were treated with L. acidophilus of MOI 100 for 48 h in HT-29 cells. The red dots in the differentially expressed gene volcano map represent up-regulated, differentially expressed genes; blue dots represent down-regulated, differentially expressed genes, and gray dots represent non-differentially expressed genes. The heatmap expresses cancer-related genes, where red represents genes with high log2 (FPKM/average FPKM) expression, and blue represents low expression.