Fig. 4.

CREs regulate ebony expression in the developing epidermis. a) Confocal fluorescence images of the developing abdominal epidermis of w¹¹¹⁸; e::mCherry. b) Confocal fluorescence images of the developing abdominal epidermis of w¹¹¹⁸; e^Δ1017priEE::mCherry, w¹¹¹⁸; e^Δ1027priEE::mCherry. c) Confocal fluorescence images of the developing abdominal epidermis of priEE-EGFP transformed to VK00033. d) Bright-field images of c). Images of females and males are shown in the upper and lower panels, respectively. The red arrowhead indicates the dorsal midline. The yellow arrowhead indicates a dark stripe on the posterior area of each tergite. The yellow bracket indicates male-specific dark pigmentation in the A5 and A6 tergites. Scale bars indicate 100 μm. e) Summary of the ebony expression sites for each strain determined from the fluorescence signals. f) The suggested model for the regulation of ebony expression in the abdomen. The solid lines indicate the effects of priEE, while the dotted lines indicate the effects of other CREs. The priEE fragment was also equipped to function as a midline silencer.