Fig. 5.

Location of the dorsal abdominal midline silencer within the priEE. a) The absence and presence of the dark pigmentation along the dorsal midline of the abdomen in w¹¹¹⁸; e^{Δ1092priEE (5'_partial)}::mCherry (left panel) and in w¹¹¹⁸; e^{Δ498priEE (3'_partial)}::mCherry (right panel), respectively. b) The absence and presence of Ebony-mCherry repression along the dorsal midline of the abdomen in w¹¹¹⁸; e^{Δ1092priEE (5'_partial)}::mCherry (left panel) and in w¹¹¹⁸; e^{Δ498priEE (3'_partial)}::mCherry (right panel), respectively. Scale bars indicate 100 μm. c) The summary of the breakpoints of the deletions and the tested fragments by reporter assays and the midline silencer activity detected in the corresponding strains. The red arrowheads indicate the positions of the gRNA target sites used to generate partial deletions of the priEE. Dotted lines indicate deleted positions. ^a and ^b are from Rebeiz et al. (2009). The double arrow indicates the position of the 351-bp fragment that includes the silencer.