Figure 6.

CD137 encompassing CARs induce the release of sCD137 on activation. (A) Antimesothelin second-generation CARs encoding cytoplasmic sequences of either CD137 or CD28 were lentivirally transduced to activated human CD8 T lymphocytes. Such resulting T cell cultures could be activated by microbeads decorated with chemically linked recombinant mesothelin-Fc. (B) sCD137 concentration in the culture of the corresponding CAR T cells stimulated or not with mesothelin-coated microbeads 24 and 48 hours following of exposure to the beads. (C) CD137 surface expression in the corresponding lymphocyte cultures. (D) IFNγ concentrations in the supernatants. Individual results from three or four independent donors whose individual values are linked by dotted lines are shown and the color-coded bars indicate the value of the means. CAR, chimeric antigen receptor.