Antibodies |
|
|
|
Anti-mouse CD3ε (145-2C11) |
BioLegend |
Cat #100203, RRID:AB_312660 |
Anti-mouse CD4 (GK1.5) |
BioLegend |
Cat #100405, RRID:AB_312690 |
Anti-mouse CD8b (YTS156.7.7) |
BioLegend |
Cat #126619, RRID:AB_2563950 |
Anti-mouse CD11b (M1/70) |
BioLegend |
Cat #101229, RRID:AB_2129375 |
Anti-mouse CD11c (N418) |
BioLegend |
Cat #117313, RRID:AB_492849 |
Anti-mouse CD25 (PC61) |
BioLegend |
Cat #102029, RRID:AB_893291 |
Anti-mouse CD44 (1M7) |
ThermoFisher |
Cat #25-0441-82, RRID:AB_469623 |
Anti-mouse CD45.1 (A20) |
BioLegend |
Cat #110713, RRID:AB_313502 |
Anti-mouse CD45.2 (104) |
BioLegend |
Cat #109829, RRID:AB_1186103 |
Anti-mouse/human B220 (RA3-6B2) |
BioLegend |
Cat #103229, RRID:AB_492875 |
Anti-mouse CXCL9 (MIG-2F5.5) |
BioXCell |
Cat #BE0309, RRID:AB_2736989 |
Anti-mouse CXCR3 (CXCR3-173) |
BioLegend |
Cat #126521, RRID:AB_10900974 |
Anti-mouse CXCR3 (CXCR3-173) |
BioXCell |
Cat #BE0249, RRID:AB_2687730 |
Anti-mouse DCIR2 (33D1) |
BioLegend |
Cat #124914, RRID:AB_1227625 |
Anti-mouse F4/80 (BM8) |
BioLegend |
Cat #123120, RRID:AB_893479 |
Anti-mouse KLRG1 (2F1/KLRG1) |
BioLegend |
Cat #138415, RRID:AB_2561735 |
Anti-mouse/human Ki67 (SolA15) |
ThermoFisher |
Cat #17-5698-82, RRID:AB_2688057 |
Anti-mouse Ly6G (1A8) |
BioLegend |
Cat #127624, RRID:AB_10640819 |
Anti-mouse MHC-II (M5/114.15.2) |
BioLegend |
Cat #107620, RRID:AB_493527 |
Anti-mouse NK1.1 (PK136) |
BioLegend |
Cat #108710, RRID:AB_313397 |
Anti-mouse/human TCF1 (C63D9) |
Cell Signaling |
Cat #2203S, RRID:AB_2199302 |
Anti-mouse/rat XCR1 (ZET) |
BioLegend |
Cat #148204, RRID:AB_2563843 |
Polyclonal anti-GFP |
ThermoFisher |
Cat #A-21311, RRID:AB_221477 |
|
Chemicals, peptides, and recombinant proteins |
|
|
|
CAS-Block Histochemical Reagent |
ThermoFisher |
Cat #008120 |
Ghost Dye Violet 510 |
Tonbo Biosciences |
Cat #13-0870-T500 |
CFSE proliferation dye |
ThermoFisher |
Cat #C34554
|
Collagenase D |
Sigma-Aldrich |
Cat #11088866001 |
Tissue-Tek O.C.T. compound |
Sakura |
Product code 4583 |
Opti-Prep |
StemCell Technologies |
Cat #07820 |
GRA6 Peptide (HPGSVNEFDF) |
Peptide 2.0 Inc. |
N/A |
|
Critical commercial assays |
|
|
|
FoxP3 staining kit |
ThermoFisher |
Cat #00-5523-00 |
CD8a+ T cell isolation kit, mouse |
Miltenyi Biotec. |
Cat #130-104-075 |
Quick-RNA Microprep Kit |
Zymo Research |
Cat #R1050 |
|
Deposited data |
|
|
|
Raw and analyzed data |
N/A |
GEO: GSE187545
|
|
Experimental models: Organisms/strains |
|
|
|
C57BL/6J |
The Jackson Laboratory |
Stock No: 000644 |
B6.c (B6.C-H2d/bByJ) |
The Jackson Laboratory |
Stock No: 000359 |
UBI-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) |
The Jackson Laboratory |
Stock No: 004353 |
CXCR3 KO B6 (B6.129P2-Cxcr3tm1Dgen/J) |
The Jackson Laboratory |
Stock No: 005796 |
REX3 B6 |
Groom et al., 2012
|
N/A |
TG6 TCR Transgenic |
Chu et al., 2016
|
N/A |
T. gondii type II Prugniuad-tomato-OVA |
Chtanova et al., 2008
|
N/A |
|
Software and algorithms |
|
|
|
FlowJo |
FlowJo LLC |
Version 10 |
DISC (Dynamic in situ cytometry) |
Moreau et al., 2012
|
N/A |
ImageJ |
(Schneider et al., 2012) |
N/A |
Imaris Software |
Bitplane Scientific |
N/A |
Trimmomatic |
Bolger et al. (2014)
|
N/A |
Bowtie2 |
Langmead and Salzberg, 2012
|
N/A |
RSEM |
Li and Dewey, 2011
|
N/A |
DESeq2 |
Love et al. (2014)
|
N/A |
FGSEA |
Korotkevich et al., 2016
|
N/A |
Prism (Version 7) |
GraphPad Software |
N/A |
RNA-seq analysis code |
N/A |
Zenodo: https://doi.org/10.5281/zenodo.5794236
|