Table 3.

Characterization of the pairings of the 609A heavy chain with a variety of light chains

Pairings		Homology to 609 LC	EC50± SD (nM)	IC50± SD (nM)	EC50 (nM)	KD (nM)	Germline
Name	LC sources	Percentage	ELISA Binding	Blocking	FACS Binding	Biacore
609	609	100.00%	0.11 ± 0.003	0.13 ± 0.016	0.38	2.37E-09	IGKV3-11*01
609HC-1LC	mAb1-LC	84.11%	0.62 ± 0.120	0.14 ± 0.010	0.65	5.46E-07	IGKV3-11*01
609HC-2LC	ipilimumab-LC, anti-CTLA4	81.48%	0.15 ± 0.004	0.11 ± 0.006	0.48	6.33E-09	IGKV3-20*01
609HC-3LC	9C10-LC, anti-PDL1	73.83%	0.19 ± 0.008	0.11 ± 0.004	0.55	2.98E-08	IGKV6-21*01
609HC-4LC	mAb4-LC	70.09%	0.24 ± 0.012	0.12 ± 0.011	0.58	2.97E-08	IGKV6-21*02
609HC-5LC	mAb5-LC	68.22%	0.15 ± 0.003	0.12 ± 0.009	0.52	1.09E-09	IGKV1-33*01
609HC-6LC	mAb6-LC	67.29%	0.17 ± 0.003	0.13 ± 0.005	0.39	1.12E-08	IGKV1-33*01
609HC-7LC	mAb7-LC	66.36%	0.72 ± 0.160	0.12 ± 0.013	0.44	8.39E-07	IGKV1-39*01
609HC-8LC	mAb8-LC	66.36%	0.22 ± 0.017	0.10 ± 0.010	0.43	2.71E-08	IGKV1-39*01
609HC-9LC	mAb9-LC	65.77%	0.15 ± 0.003	0.11 ± 0.012	0.35	1.24E-08	IGKV1-39*01
609HC-10LC	mAb10-LC	63.89%	NA	NA	NA	NA	IGKV1-39*01
609HC-11LC	mAb11-LC	59.82%	0.65 ± 0.121	0.12 ± 0.014	0.33	6.59E-07	IGKV2-30*01
609HC-12LC	mAb12-LC	59.43%	1.04 ± 0.121	0.17 ± 0.008	1.03	4.87E-07	IGKV1-33*01