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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cell. 2022 Feb 3;185(3):513–529.e21. doi: 10.1016/j.cell.2022.01.002

Figure 2. Strain-level fitness in the Bacteroides is dependent on the interactions of sugars and butyrate.

Figure 2.

(A) Heatmap of sensitivity to butyrate as a function of monosaccharide utilization (See STAR Methods for details on inhibition scoring). Row presents the phylogenic tree of the Bacteroides and Parabacteroides.

(B–E) Strains as indicated were grown in monosaccharides (B), human milk oligosaccharides (C), glycans (D), and monosaccharides and cognate homodimers disaccharides (E) with and without butyrate. See also Figures S1D and S1E.

(F) B. nordii CL02, B. ovatus ATCC, B. salyersiae DSM, and B. caccae ATCC grown in inulin or pectin −/+ butyrate.

(G) Schematic of gnotobiotic experiments (top). Log fold change of relative abundance (post/pre butyrate in drinking water for given dietary glycan) of Bn CL02, Bo ATCC, Bs DSM, and Bc ATCC (bottom). p value by two-tailed Student’s t test as indicated (n = 5/group). See STAR Methods for details.

Shown is a representative of two biological replicates in (A–F).