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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cell. 2022 Feb 3;185(3):513–529.e21. doi: 10.1016/j.cell.2022.01.002

Figure 5. Synergy in Acyl-CoA metabolism interacts with BCAAs to tune sugar-dependent inhibitory effects of butyrate.

Figure 5.

(A) Tn-seq of Bv Δ1163 grown in Gal −/+ butyrate. Two operons, BVU_0575/0574 and BVU_2720/2719, are highlighted in green. Fold change and p value were calculated by combining two biological replicates. See also Table S3.

(B) Genomic locus of BVU_0575/0574 encoding transcriptional regulator and Acetyl-CoA synthetase, and BVU_2720/2719 encoding transcriptional regulator and Acyl-CoA synthetase and predicted enzymatic reactions.

(C) Bv WT and isogenic strains with deletions in Acyl-CoA transferase (BVU_1163) and Acyl-CoA synthetases (BVU_2719, BVU_0574) grown in Glc or Gal −/+ butyrate.

(D) Bt Δ3942 and Bv WT grown in Glc supplemented with Val, Leu, or Ile −/+ butyrate.

(E and F) Bv WT and isogenic strains with deletions in Acyl-CoA transferase (BVU_1163) and Acyl-CoA synthetases (BVU_2719, BVU_0574) (E) and natural human gut Bacteroides strains (F) grown in Glc or Gal supplemented with Val, Leu, or Ile −/+ butyrate.

Shown is a representative of three biological replicates in (C–F).