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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cell. 2022 Feb 3;185(3):513–529.e21. doi: 10.1016/j.cell.2022.01.002

Figure 6. Species-level genetic variation in Acyl-CoA metabolic defense among the Bacteroides.

Figure 6.

(A) Protein identity of Acyl-CoA thioesterases, BT_3942 (Bt VPI), and BVU_0767 (Bv ATCC). See also Figures S5A and S5B.

(B) Bt Δ3942 and Bv WT complemented with BT_3942 or BVU_0767 grown in Glc −/+ butyrate.

(C) Acyl-CoA thioesterase activity of the recombinant proteins, BT_3942, and BVU_0767 with different chain lengths of acyl-CoAs as substrate. See also Figure S5C.

(D) Protein identity of Acyl-CoA transferases, BVU_1163 (Bv ATCC) and BT_3193 (Bt VPI). See also Figures S5D and S5E.

(E) Bv Δ1163 and Bt WT complemented with BVU_1163 or BT_3193 grown in Glc −/+ butyrate.

(F) Relative mRNA expression of Acyl-CoA transferase in Bt VPI (BT_3193) and Bv ATCC (BVU_1163) grown in Glc across growth phases. See also Figure S5F.

(G) mRNA levels of Acyl-CoA transferase genes (left) and inhibitory effect of butyrate (right) in Bv Δ1163 strains harboring plasmids expressing BVU_1163 or BT_3193 under their native or swapped promoters.

(H) Schematic of RNA polymerase binding sites showing nucleotide polymorphisms within the promoter regions of BVU_1163 and BT_3193.

(I) Schematic of the nucleotide polymorphism constructs on pBv and pBt promoter backbones (left), mRNA levels of BVU_1163 (middle), and inhibitory effect of butyrate (right) in Bv Δ1163 strains harboring plasmids driving BVU_1163 under the promoter with each strain-specific nucleotide polymorphism.

Error bars represent mean ± SEM of two biological replicates in (C and F) and three biological replicates in (G and I). Nonsignificant p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001; unpaired two-tailed t test in (C, F, G, and I), One-Way ANOVA with Dunnett multiple comparisons test in (C, red asterisks). Shown is a representative of three biological replicates in (B, E, G, and I).