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. Author manuscript; available in PMC: 2022 Mar 4.
Published in final edited form as: Bioorg Med Chem Lett. 2021 Oct 16;53:128414. doi: 10.1016/j.bmcl.2021.128414

Figure 2. Palmostatin analog effects on the dynamic palmitoylation of N-Ras.

Figure 2.

a, b, Effect of active (12) and inactive (1) palmostatin analogs on N-Ras (a, b) and global protein (a) palmitoylation as detected by pulse-chase metabolic labeling of ON cells (or ONK control cells) with the 17-ODYA probe. Data shown are representative of three independent experiments. For b, N-Ras was immunoprecipitated using anti-GFP antibodies prior to visualizing palmitoylation by CuAAC conjugation to a Rh-N3 reporter tag (top panel). Total N-Ras content was measured by Western blotting of GFP enrichments (bottom panel).c, Quantification of palmostatin analog effects on N-Ras palmitoylation; data represent average values ± s.d. from three independent biological replicates, where densitometry was performed on N-Ras signals from anti-GPF immunoprecipitations as shown in b (top panel).