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. Author manuscript; available in PMC: 2022 Mar 4.
Published in final edited form as: Bioorg Med Chem Lett. 2021 Oct 16;53:128414. doi: 10.1016/j.bmcl.2021.128414

Figure 3. Concentration dependent effects of palmostatin analog 12 on N-Ras and global protein palmitoylation.

Figure 3.

a, Effect of 12 on N-Ras (a, b) and global protein (a) palmitoylation as detected by pulse-chase metabolic labeling of ON cells (or ONK control cells) with the 17-ODYA probe. Data shown are representative of three independent experiments. For b, N-Ras was immunoprecipitated using anti-GFP antibodies prior to visualizing palmitoylation by CuAAC conjugation to a Rh-N3 reporter tag (top panel). Total N-Ras content was measured by Western blotting of GFP enrichments (bottom panel). c, Quantification of concentration-dependent effects of 12 on N-Ras palmitoylation; data represent average values ± s.d. from two independent biological replicates, where densitometry was performed on N-Ras signals from anti-GFP immunoprecipitations as shown in b (top panel).